Journal article
Molecular Profiling of VGluT1 AND VGluT2 Ventral Subiculum to Nucleus Accumbens Shell Projections
S Jin, EJ Campbell, CK Ip, S Layfield, RAD Bathgate, H Herzog, AJ Lawrence
Neurochemical Research | SPRINGER/PLENUM PUBLISHERS | Published : 2023
Abstract
The nucleus accumbens shell is a critical node in reward circuitry, encoding environments associated with reward. Long-range inputs from the ventral hippocampus (ventral subiculum) to the nucleus accumbens shell have been identified, yet their precise molecular phenotype remains to be determined. Here we used retrograde tracing to identify the ventral subiculum as the brain region with the densest glutamatergic (VGluT1–Slc17a7) input to the shell. We then used circuit-directed translating ribosome affinity purification to examine the molecular characteristics of distinct glutamatergic (VGluT1, VGluT2–Slc17a6) ventral subiculum to nucleus accumbens shell projections. We immunoprecipitated tra..
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Awarded by National Institutes of Health
Funding Acknowledgements
This work was supported by a National Health and Medical Research Council Project Grant to AJL and EJC (1159032) and a National Health and Medical Research Council Synergy Grant to AJL (2009851), a Brain and Behavior Research Foundation NARSAD Young Investigator Grant to EJC (28007) and a University of Melbourne Early Career Researcher Grant to EJC. We acknowledge the Victorian State Government Operational Infrastructure Scheme. This work was also supported in part through the NIH/NCI Cancer Center Support Grant Grant P30 CA008748 which funds The Rockefeller University Bi-Institutional Antibody and Bioresource Core Facility