Journal article
SERS Endoscopy for Monitoring Intracellular Drug Dynamics
B Fortuni, M Ricci, R Vitale, T Inose, Q Zhang, JA Hutchison, K Hirai, Y Fujita, S Toyouchi, S Krzyzowska, I Van Zundert, S Rocha, H Uji-I
ACS Sensors | AMER CHEMICAL SOC | Published : 2023
Abstract
Understanding the dynamics and distribution of medicinal drugs in living cells is essential for the design and discovery of treatments. The tools available for revealing this information are, however, extremely limited. Here, we report the application of surface-enhanced Raman scattering (SERS) endoscopy, using plasmonic nanowires as SERS probes, to monitor the intracellular fate and dynamics of a common chemo-drug, doxorubicin, in A549 cancer cells. The unique spatio-temporal resolution of this technique reveals unprecedented information on the mode of action of doxorubicin: its localization in the nucleus, its complexation with medium components, and its intercalation with DNA as a functio..
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Awarded by China Scholarship Council
Funding Acknowledgements
KAKENHI (23H04877, 22H00328, 22K20524, 21K18192, 21H04634, 20K05413, 19KK0136), Research Foundation of Flanders (FWO) research grant (G0D4519N, G081916N, 12X1419N, and 12X1423N), ERC (#280064 to H.U.-i.), KU Leuven (C14/15/053, C14/19/079), BELSPO (IAP VII/05), JST PRESTO (JPMJPR2104), Australian Research Council (ARC) Future Fellowship (FT180100295), ARC Centre of Excellence in Exciton Science (CE170100026), JSPS Core-to -Core (Program A).