Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures

J Wang; M Daniszewski; MM Hao; D Hernández; A Pébay; PA Gleeson; L Fourriere

Journal article

Organelle mapping in dendrites of human iPSC-derived neurons reveals dynamic functional dendritic Golgi structures

J Wang, M Daniszewski, MM Hao, D Hernández, A Pébay, PA Gleeson, L Fourriere

Cell Reports | Published : 2023

DOI: 10.1016/j.celrep.2023.112709

Abstract

Secretory pathways within dendrites of neurons have been proposed for local transport of newly synthesized proteins. However, little is known about the dynamics of the local secretory system and whether the organelles are transient or stable structures. Here, we quantify the spatial and dynamic behavior of dendritic Golgi and endosomes during differentiation of human neurons generated from induced pluripotent stem cells (iPSCs). In early neuronal development, before and during migration, the entire Golgi apparatus transiently translocates from the soma into dendrites. In mature neurons, dynamic Golgi elements, containing cis and trans cisternae, are transported from the soma along dendrites,..

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University of Melbourne Researchers

Maciej Daniszewski Author

Marlene Hao Author

Alice Pebay Author

Paul Gleeson Author

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Grants

Awarded by Australian Government

Funding Acknowledgements

& nbsp;This work was supported by funding from NHMRC Australia (APP1163862) . J.W. is supported by a University of Melbourne International Postgraduate Award. A.P. is supported by an NHMRC Australia Senior Research Fellowship (APP1154389) . The authors acknowledge the facilities and the scientific and technical assistance of the iPSC reprogramming facility, the Stem Cell Disease Modeling laboratory, The University of Melbourne, which is supported by Phe- nomics Australia (PA) . A.P. is supported by the Australian Government through the National Collaborative Research Infrastructure Strategy (NCRS) program. Confocal microscopy and image processing were performed at the Biological Optical Microscopy Platform (BOMP) at the University of Melbourne. We acknowledge Fiona Houghton for excellent laboratory support.