The Transition from Resin Chromatography to Membrane Adsorbers for Protein Separations at Industrial Scale

Abstract

Membrane adsorbers (MA) are emerging as alternative unit operations in high-value protein separation due to their many benefits, including short residence times, low pressure drop, ease of scale-up and cost-effectiveness. Historically, the MA literature has been directed to new membrane materials and their use as a polishing step due to their low binding capacity. More recently, the emergence of membrane bind-elute modalities has extended their application at industrial scale, and a move from established resin chromatography to MA is feasible. To fill this gap and speed the development of MA, a review on the purification of monoclonal antibodies and other proteins in the pharmaceutical and f..