Journal article
Murine Norovirus: Additional Protocols for Basic and Antiviral Studies
CE Wobus, AM Peiper, AM McSweeney, VL Young, M Chaika, MS Lane, M Lingemann, JM Deerain, MS Strine, MM Alfajaro, EW Helm, SM Karst, JM Mackenzie, S Taube, VK Ward, CB Wilen
Current Protocols | Published : 2023
DOI: 10.1002/cpz1.828
Abstract
Murine norovirus (MNV) is a positive-sense, plus-stranded RNA virus in the Caliciviridae family. Viruses in this family replicate in the intestine and are transmitted by the fecal-oral route. MNV is related to the human noroviruses, which cause the majority of nonbacterial gastroenteritis worldwide. Given the technical challenges in studying human norovirus, MNV is often used to study mechanisms in norovirus biology since it combines the availability of a cell culture and reverse genetics system with the ability to study infection in the native host. Adding to our previous protocol collection, here we describe additional techniques that have since been developed to study MNV biology. © 2023 ..
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Awarded by National Institutes of Health
Funding Acknowledgements
C.E.W. was supported by NIH R21 AI 154647, S.M.K. by NIH R01AI162970 and R01AI141478, C.B.W. by NIH R01 AI148467, S.T. by the DFG research unit VIROCARB (FOR2327, TA1093-2-2), A.M.P. by NIH T32AI007110, and E.W.H. by NIH F30AI154834. Research in the lab of V.W. was funded by the New Zealand Ministry for Business Innovation and Employment, grant number UOOX1904. J.M.M. was funded by the National Health and Medical Research Council of Australia, grant number 1123135, and the Miller Foundation.