Journal article
High Resolution Proteomic Analysis of Subcellular Fractionated Boar Spermatozoa Provides Comprehensive Insights Into Perinuclear Theca-Residing Proteins
M Zhang, RZ Chiozzi, DA Skerrett-Byrne, T Veenendaal, J Klumperman, AJR Heck, B Nixon, JB Helms, BM Gadella, EG Bromfield
Frontiers in Cell and Developmental Biology | Published : 2022
Abstract
The perinuclear theca (PT) is a highly condensed, largely insoluble protein structure that surrounds the nucleus of eutherian spermatozoa. Recent reports have indicated that the PT unexpectedly houses several somatic proteins, such as core histones, which may be important post-fertilization during re-modelling of the male pronucleus, yet little is known regarding the overall proteomic composition of the PT. Here, we report the first in depth, label-free proteomic characterization of the PT of boar spermatozoa following the implementation of a long-established subcellular fractionation protocol designed to increase the detection of low abundance proteins. A total of 1,802 proteins were identi..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
MZ is financed by the China Scholarship Council (CSC) (No. 201606170117). EB is recipient of an NHMRC CJ Martin Early Career Fellowship (APP1138701). The proteomics analysis was done by RC at the Netherlands Proteomics Centre and partially financed by the National Roadmap for Large-Scale Research Infrastructure of the Dutch Research Council NWO through the X-omics Road Map program (Project 184.034.019 to AH) and the EU Horizon 2020 program Epic-XS (Project 823839 to RC and AH). The electron microscopy was done in the Cell Microscopy Core of the University Medical Centre Utrecht and partially financed by the National Roadmap for Large-Scale Research Infrastructure of the Dutch Research Council NWO (NEMI; project number 184.034.014 to JK).