Journal article
Serological assays to measure dimeric IgA antibodies in SARS-CoV-2 infections
Z Wei, F Angrisano, EM Eriksson, R Mazhari, H Van, S Zheng, RJ Center, I Boo, J McMahon, J Lau, N Kiernan-Walker, S Ruybal-Pesántez, I Mueller, LJ Robinson, DA Anderson, HE Drummer
Immunology and Cell Biology | WILEY | Published : 2023
DOI: 10.1111/imcb.12682
Abstract
Current serological tests cannot differentiate between total immunoglobulin A (IgA) and dimeric IgA (dIgA) associated with mucosal immunity. Here, we describe two new assays, dIgA-ELISA and dIgA-multiplex bead assay (MBA), that utilize the preferential binding of dIgA to a chimeric form of secretory component, allowing the differentiation between dIgA and monomeric IgA. dIgA responses elicited through severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection were measured in (i) a longitudinal panel, consisting of 74 samples (n = 20 individuals) from hospitalized cases of coronavirus disease 2019 (COVID-19); (ii) a longitudinal panel, consisting of 96 samples (n = 10 individuals..
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Awarded by National Health and Medical Research Council
Funding Acknowledgements
The authors gratefully acknowledge the contribution to this work of the Victorian Operational Infrastructure Support Program received by the Burnet Institute. This study was supported by the COVID-19 Victorian Consortium grant from the Victorian Government. HV was supported by an APPRISE Research Fellowship from the National Health and Medical Research Council of Australia (NHMRC; 1116530). FA was supported by the IMPACT Philanthropy Application Program and a Burnet Institute Beever Fellowship. We thank Jessie Kingston, Sarah Hess and Stephanie Routley for technical assistance and Khayriyyah Mohd Hanafiah for technical discussions. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.