Journal article
Molecular basis of sulfolactate synthesis by sulfolactaldehyde dehydrogenase from Rhizobium leguminosarum
J Li, M Sharma, R Meek, A Alhifthi, Z Armstrong, NM Soler, M Lee, ED Goddard-Borger, JN Blaza, GJ Davies, SJ Williams
Chemical Science | ROYAL SOC CHEMISTRY | Published : 2023
DOI: 10.1039/d3sc01594g
Open access
Abstract
Sulfolactate (SL) is a short-chain organosulfonate that is an important reservoir of sulfur in the biosphere. SL is produced by oxidation of sulfolactaldehyde (SLA), which in turn derives from sulfoglycolysis of the sulfosugar sulfoquinovose, or through oxidation of 2,3-dihydroxypropanesulfonate. Oxidation of SLA is catalyzed by SLA dehydrogenases belonging to the aldehyde dehydrogenase superfamily. We report that SLA dehydrogenase RlGabD from the sulfoglycolytic bacterium Rhizobium leguminsarum SRDI565 can use both NAD+ and NADP+ as cofactor to oxidize SLA, and indicatively operates through a rapid equilibrium ordered mechanism. We report the cryo-EM structure of RlGabD bound to NADH, revea..
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Awarded by Walter and Eliza Hall Institute of Medical Research
Funding Acknowledgements
This work was supported by the Australian Research Council (DP210100233, DP210100235), the Biotechnology and Biological Sciences Research Council (BB/W003805/1), the UKRI Future Leader Fellowship Program (MR/T040742/1), and the Royal Society for the Ken Murray Research Professorship to G. J. D. and the associated PDRA funding (RP\EA\180016) for RWM. J. L. is supported by the China Scholarship Council. E. D. G.-B. acknowledges support from The Walter and Eliza Hall Institute of Medical Research, National Health and Medical Research Council of Australia (NHMRC) project grant GNT2000517, the Australian Cancer Research Fund, and the Brian M. Davis Charitable Foundation Centenary Fellowship. We thank the Wellcome Trust for funding the Glacios electron microscope (grant number 206161/Z/17/Z) and Dr Johan Turkenburg and Sam Hart for assistance with cryo-EM data collection. We acknowledge Dr Andrew Leech at the University of York Bioscience Technology Facility for assistance with SEC-MALLS analysis, and Arashdeep Kaur for assistance with bioinformatics.