Journal article
Real-time, label-free detection and identification of bacteria through non-invasive optical imaging
Farah Qazi, Rajni Verma, Connagh E Redmond, Asma Khalid, Neil M O'Brien-Simpson, Snjezana Tomljenovic-Hanic
Microbes and Infection: a journal on infectious agents and host defenses | Elsevier | Published : 2024
Abstract
Currently, traditional and newer molecular and mass spectrometry techniques of identifying bacteria from biological samples requires lengthy sample preparation, growth and labelling/staining assays. Thus, there is a pressing clinical need for an adjunct method that accurately identifies bacteria in real time. Here we report on the evaluation of confocal microscopy for the identification of clinically important and multi-drug resistant (MDR) bacteria in real time, using their intrinsic fluorescence features, i.e., emission spectra and fluorescence lifetime. The results demonstrate that difference in emission spectra and fluorescence lifetimes can be used as a fingerprint for identification of..
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Awarded by NHMRC
Awarded by ARC
Awarded by Cancer Council Victoria
Funding Acknowledgements
F.Q. acknowledges University of Melbourne Research Scholarship. R.V. acknowledges University of Melbourne McKenzie Fellowship. This project was partly funded by Defence Technologies Seed Grant at the University of Melbourne. Support from Prof. Steven Prawer at University of Melbourne is greatly acknowledged. The authors are thankful to Dr. Brian Yang from RMIT University for his assistance with room temperature confocal microscopy. Zeiss LSM 880 confocal microscopy experiments were performed at the Biological Optical Microscopy (BOMP) platform at the University of Melbourne. We are thankful to Dr. Jean Philippe Tetienne for valuable discussion on lifetime.The National Health and Medical Research Council (NHMRC) of Australia and Australian Research Council (ARC) are thanked for financial support over many years for the microbiology and chemical biology studies reported in the authors'laboratories. NMOS is the recipient of NHMRC funding (APP1142472, APP1158841, APP1185426) , ARC funding (DP160101312, LE200100163) , Cancer Council Victoria funding (APP1163284) and Australian Dental Research Funding in microbial materials and research is supported by the Centre for Oral Health Research at The Melbourne Dental School. CER is supported by NHMRC funding (APP1185426) , and their microbial materials and research is supported by the Centre for Oral Health Research at The Melbourne Dental School.r Biological Optical Microscopy (BOMP) platform at the University of Melbourne. We are thankful to Dr. Jean Philippe Tetienne for valuable discussion on lifetime. The National Health and Medical Research Council (NHMRC) of Australia and Australian Research Council (ARC) are thanked for financial support over many years for the microbiology and chemical biology studies reported in the authors'laboratories. NMOS is the recipient of NHMRC funding (APP1142472, APP1158841, APP1185426) , ARC funding (DP160101312, LE200100163) , Cancer Council Victoria funding (APP1163284) and Australian Dental Research Funding in microbial materials and research is supported by the Centre for Oral Health Research at The Melbourne Dental School. CER is supported by NHMRC funding (APP1185426) , and their microbial materials and research is supported by the Centre for Oral Health Research at The Melbourne Dental School.