Journal article
Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex-Forming Oligonucleotide
Sukhvir Kaur Bhangu, Liviana Mummolo, Soraia Fernandes, Alessia Amodio, Agata Radziwon, Brendan Dyett, Marco Savioli, Nitin Mantri, Christina Cortez-Jugo, Frank Caruso, Francesca Cavalieri
Advanced Functional Materials | Wiley | Published : 2024
Abstract
Nanoparticle-mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and the intracellular trafficking of the engineered nanoparticles. Unravelling the molecular arrangements of oligonucleotides within the nanoparticles as well as their intracellular behavior are essential for designing effective nucleic acid delivery systems. Herein, a simple and general strategy for probing the endosomal escape of nanoparticles carrying oligonucleotides in live cells is reported. A triplex-forming oligonucleotide probe is designed to target the transcription factor, kappa-light-chain-enhancer of activated B cells (NF-κB), in the cytosol of cells and to t..
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Grants
Awarded by National Health and Medical Research Council Leadership Fellowship
Awarded by European Union's Horizon 2020 research and innovation program under the Marie Sklstrok;odowska-Curie
Funding Acknowledgements
F.Cavalieri acknowledges the award of an RMIT Vice Chancellor Senior Research Fellowship and F.Caruso acknowledges the award of a National Health and Medical Research Council Leadership Fellowship (GNT2016732). This work was supported by the Australian Research Council under a Discovery Project scheme (F. Cavalieri, C.C.-J., and N.M., DP210101792). This work received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sk & lstrok;odowska-Curie grant agreement no. 690901 ("NANOSUPREMI"). This work was performed in part at the Materials Characterization and Fabrication Platform (MCFP) and Bio21 Ian Holmes Imaging Center at The University of Melbourne. The authors acknowledge Haiyan Zhun and Domitilla Vanni for providing help with TEM and NMR measurements.