Ultrastructural and glycoproteomic characterization of Prevotella intermedia: Insights into O-glycosylation and outer membrane vesicles

X Ye; B Paul; J Mo; EC Reynolds; D Ghosal; PD Veith

Journal article

Ultrastructural and glycoproteomic characterization of Prevotella intermedia: Insights into O-glycosylation and outer membrane vesicles

X Ye, B Paul, J Mo, EC Reynolds, D Ghosal, PD Veith

MicrobiologyOpen | Wiley | Published : 2024

DOI: 10.1002/mbo3.1401

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Abstract

Prevotella intermedia, a Gram-negative bacterium from the Bacteroidota phylum, is associated with periodontitis. Other species within this phylum are known to possess the general O-glycosylation system. The O-glycoproteome has been characterized in several species, including Tannerella forsythia, Porphyromonas gingivalis, and Flavobacterium johnsoniae. In our study, we used electron cryotomography (cryoET) and glycoproteomics to reveal the ultrastructure of P. intermedia and characterize its O-glycoproteome. Our cryoET analysis unveiled the ultrastructural details of the cell envelope and outer membrane vesicles (OMVs) of P. intermedia. We observed an electron-dense surface layer surrounding..

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University of Melbourne Researchers

Winnie Ye Author

Eric Reynolds Author

Debnath Ghosal Author

Paul Veith Author

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Funding Acknowledgements

We thank the staff of the Mass Spectrometry and Proteomics Facility at the Bio21 Institute, University of Melbourne, Australia for the acquisition of Orbitrap LC-MS/MS data and related technical support. This work was supported by the Australian National Health and Medical Research Council grant ID 1123866, the Australian Research Council grant DP200100914, and the Australian Government Department of Industry, Innovation, and Science Grant ID 20080108. DG is supported by an NHMRC grant (APP1196924 to DG), an HFSP grant (RGEC33/2023), and an Australia-India Strategic Research Fund (AIRXIV000108). Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.