Constitutive Flt3 signaling impacts conventional dendritic cell function

KR Wilson; C Macri; JA Villadangos; JD Mintern

Journal article

Constitutive Flt3 signaling impacts conventional dendritic cell function

KR Wilson, C Macri, JA Villadangos, JD Mintern

Immunology and Cell Biology | WILEY | Published : 2024

DOI: 10.1111/imcb.12757

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Abstract

The development of dendritic cells (DCs) depends on signaling via the FMS-like tyrosine kinase 3 (Flt3) receptor. How Flt3 signaling impacts terminally differentiated DC function is unknown. This is important given the increasing interest in exploiting Flt3 for vaccination and tumor immunotherapy. Here, we examined DCs in mice harboring constitutively activated Flt3 (Flt3-ITD). Flt3ITD/ITD mice possessed expanded splenic DC subsets including plasmacytoid DC, conventional DC (cDC)1, cDC2, double positive (DP) cDC1 (CD11c+ CD8+ CD11b− CD103+ CD86+), noncanonical (NC) cDC1 (CD11c+ CD8+ CD11b− CD103− CD86−) and single positive (SP) cDC1 (CD11c+ CD8+ CD11b− CD103− CD86+). Outcomes of constitutive..

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University of Melbourne Researchers

Kayla Wilson Author

CHRISTOPHE MACRI Author

Jose Villadangos Author

Justine Mintern Author

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Funding Acknowledgements

The authors acknowledge critical evaluation of the manuscript by P Beavis, S Naik and C Audiger. This work was supported by Department of Health Australia, National Health and Medical Research Council grants or fellowships 1058193, 1113293, 1154502 and 1163090 (to JAV) and 1161101 and 1129672 (to JDM), Department of Education and Training, Australian Research Council (ARC) grants or fellowships 160103134, 170102471 and 190102213 (to JAV) and 190101242, 180100844, 160101373 and 180100521 (to JDM), a Human Frontiers Science Program grant (0064/2011 to JAV), and the Australian Government's National Health and Medical Research Council Victorian State Government Operational Infrastructure Support and the Independent Research Institutes Infrastructure Support Scheme. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.