Functionalized Collagen I Membranes as a Bruch’s Membrane Mimetic for Outer Retinal In Vitro Models

Abstract

Physiologically relevant in vitro models of the human outer retina are required to better elucidate the complex interplay of retinal tissue layers and investigate their role in retinal degenerative disorders. Materials currently used to mimic the function of Bruch’s membrane fail to replicate a range of important structural, mechanical, and biochemical properties. Here, we detail the fabrication of a surface-functionalized, fibrous collagen I membrane. We demonstrate its ability to better replicate a range of important material properties akin to the function of human Bruch’s membrane when compared with a commonly utilized synthetic polyethylene terephthalate alternative. We further reveal t..