Journal article
Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster
JL Scanlan, C Robin
G3 Genes Genomes Genetics | OXFORD UNIV PRESS INC | Published : 2024
Abstract
Ecdysteroids are major hormones in insects and control molting, growth, reproduction, physiology, and behavior. The biosynthesis of ecdysteroids such as 20-hydroxyecdysone (20E) from dietary sterols is well characterized, but ecdysteroid catabolism is poorly understood. Ecdysteroid kinases (EcKs) mediate the reversible phosphorylation of ecdysteroids, which has been implicated in ecdysteroid recycling during embryogenesis and reproduction in various insects. However, to date, only 2 EcK-encoding genes have been identified, in the silkworm Bombyx mori and the mosquito Anopheles gambiae. Previously, we identified 2 ecdysteroid kinase-like (EcKL) genes—Wallflower (Wall) and Pinkman (pkm)—in the..
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Awarded by University of Melbourne
Funding Acknowledgements
The authors would like to thank Simon Bullock for the pCFD6 plasmid, BangloreFly at The National Centre for Biological Services, the Drosophila Genomics Resource Center (NIH 2P40OD010949) for the UAS-ORF plasmids, and Philip Batterham, Michael Murray, Michael O'Connor, and the Bloomington Drosophila Stock Center (NIH P40OD018537) for Drosophila stocks. Information available at FlyBase (NIH U41HG000739) was also used extensively during this study.