Journal article
Discovery of NRG1-VII: the myeloid-derived class of NRG1
MA Berrocal-Rubio, YDJ Pawer, M Dinevska, R De Paoli-Iseppi, SS Widodo, J Gleeson, N Rajab, W De Nardo, J Hallab, A Li, T Mantamadiotis, MB Clark, CA Wells
BMC Genomics | BMC | Published : 2024
Abstract
The growth factor Neuregulin-1 (NRG1) has pleiotropic roles in proliferation and differentiation of the stem cell niche in different tissues. It has been implicated in gut, brain and muscle development and repair. Six isoform classes of NRG1 and over 28 protein isoforms have been previously described. Here we report a new class of NRG1, designated NRG1-VII to denote that these NRG1 isoforms arise from a myeloid-specific transcriptional start site (TSS) previously uncharacterized. Long-read sequencing was used to identify eight high-confidence NRG1-VII transcripts. These transcripts presented major structural differences from one another, through the use of cassette exons and alternative stop..
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Grants
Awarded by University of Melbourne
Funding Acknowledgements
The authors thank Professor Matt Watt (Department of Anatomy and Physiology, University of Melbourne), for gifting adipose tissue samples. The authors thanks Dr. Le Christy Ying and Prof. Paul Hertzog (Hudson Institute, Clayton, Melbourne) for gifting 2D macrophage RNA for this study. The authors thank Mrs. Zahra Elahi (Department of Anatomy and Physiology, University of Melbourne) for her logistic and emotional support, crucial to this study. The authors thank Mr. Yidi Deng (Department of Maths and Statistics, University of Melbourne) for discussions about statistical approaches.