Journal article
Bovine tracheal organoids for studying Mycoplasma bovis respiratory infections.
Chintha K Premachandre, Pin Shie Quah, Bang Manh Tran, Elizabeth Vincan, Georgia Deliyannis, Chinn Yi Wong, Andrés Diaz-Méndez, David C Jackson, Patrick C Reading, Glenn F Browning, Paola K Vaz, Nadeeka K Wawegama
Veterinary Microbiology | Elsevier | Published : 2025
Abstract
In vitro three-dimensional organoid models simulate key aspects of the structure and function of in vivo organs and have been used to study physiology, host-pathogen interactions, pathogenesis and pharmacodynamics. Although most organoid studies have been developed using human or mouse tissues, recent advancements have enabled the establishment of intestinal and respiratory tract organoids from domestic animal samples. Mycoplasma bovis causes chronic respiratory tract infections in cattle with significant health and economic consequences. The pathogenesis and virulence factors of M. bovis have been studied in several in vitro infection models, but the use of organoids has not been examined p..
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Awarded by University of Melbourne
Funding Acknowledgements
We are grateful to Dr. Neil Charman (Gippsland Research Station Pty Ltd.) for his veterinary expertise and guidance. The authors thank Mr. Zlatan Trifunovic and Dr. Hamish Brown of the Ian Holmes Imaging Centre, The University of Melbourne, for their assistance in sample processing and TEM image acquisition. This study was funded by the National Health and Medical Research Council (NHMRC) Ideas grant (APP1181580) awarded to B.M.T. and E.V, Australian Research Council Discovery Project grant (DP200102090) awarded to D.C.J, P.C.R and G. D. and Australian Research Council (ARC) Discovery Early Career Researcher grant (DE210101063) awarded to N. K. W.. C. K. P was supported by Melbourne International Fee Remission, Melbourne Research and Rowden White Scholarships.