Journal article

Assessing the predicted impact of single amino acid substitutions in calmodulin for CAGI6 challenges

P Turina, G Dal Cortivo, CA Enriquez Sandoval, E Alexov, DB Ascher, G Babbi, C Bakolitsa, R Casadio, P Fariselli, L Folkman, A Kamandula, P Katsonis, D Li, O Lichtarge, PL Martelli, SK Panday, DEV Pires, S Portelli, F Pucci, CHM Rodrigues Show all

Human Genetics | Published : 2025

DOI: 10.1007/s00439-024-02720-y

Abstract

Recent thermodynamic and functional studies have been conducted to evaluate the impact of amino acid substitutions on Calmodulin (CaM). The Critical Assessment of Genome Interpretation (CAGI) data provider at University of Verona (Italy) measured the melting temperature (Tm) and the percentage of unfolding (%unfold) of a set of CaM variants (CaM challenge dataset). Thermodynamic measurements for the equilibrium unfolding of CaM were obtained by monitoring far-UV Circular Dichroism as a function of temperature. These measurements were used to determine the Tm and the percentage of protein remaining unfolded at the highest temperature. The CaM challenge dataset, comprising a total of 15 single..

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University of Melbourne Researchers

Keywords

3102 Bioinformatics and Computational Biology
31 Biological Sciences