Should culture media for microphysiological systems be nutritionally physiological?

Abstract

The incipient use of microphysiological systems (MPSs) in the drug regulatory pathway is based on equivalent or superior predictive value in the evaluation of drug efficacy, safety and toxicology, compared with other novel alternative methods (NAMs) or animal studies. Current MPS practice is not deploying available physiological culture media solutions. Will the clinical translational reliability of MPSs be potentially compromised by the use of hyper-nutritional media?