An abundant 39 kDa protein from Brassica napus (canola) is present in the apoplast of leaves infected by the blackleg fungus, Leptosphaeria maculans

Abstract

An abundant 39 kDa protein was detected in the apoplast of leaves 12 days after infection of Brassica napus L. (canola) by the blackleg fungus, Leptosphaeria maculans (Desm.) Ces. & de Not. This protein was also present 24 h after leaves were sprayed with 5 mM cupric chloride, indicating that it is produced by B. napus in response to stress. Tryptic peptides cleaved from this protein had almost identical amino acid sequence to part of a 1,3-ß-glucanase from B. rapa.