Distance dependence of photoinduced electron transfer along α-Helical polypeptides

Abstract

α-Helical polypeptides containing a pair of L-1-pyrenylalanine and L-p-nitrophenylalanine that are separated by 0-8 amino acid units were synthesized. The rates of photoinduced electron transfer (ET) from the pyrenyl group to the nitrophenyl group were evaluated from the decay curves of pyrenyl fluorescence recorded at different temperatures from -58 to +30°C. The rate constants showed a complex dependence on the number of spacer amino acids. In particular, recoveries of the ET rates with increasing the number of spacer amino acids from 1 to 2 and from 5 to 6 were found. The ET rate constants, however, exhibited a simple exponential dependence on the edge-to-edge distance between the two chr..