Identification, distribution, and expression of angiotensin II receptors in the normal human prostate and benign prostatic hyperplasia

Abstract

The tissue distribution, cellular localization, and level of expression of angiotensin II (Ang II) receptors were examined in the normal human prostate and benign prostatic hyperplasia (BPH) by in vitro autoradiography, immunohistochemistry, and radioligand binding studies. In the normal human prostate, Ang II receptors were of the AT1 subtype and localized predominantly to periurethral stromal smooth muscle. The AT1 receptor antagonist losartan totally displaced specific [125I]-[Sar1,Ile8]Ang II binding, in a concentration-dependent manner, whereas the AT2receptor antagonist PD123319 was without effect. There was no significant difference in receptor affinity, but AT1 receptor density was m..