Journal article

Stimulating effect of an arteriovenous shunt on the in vivo growth of isografted fibroblasts: A preliminary report

RA Mian, KR Knight, AJ Penington, JV Hurley, A Messina, R Romeo, WA Morrison

TISSUE ENGINEERING | MARY ANN LIEBERT INC PUBL | Published : 2001

Abstract

Isogenous fibroblasts derived from the skin of inbred Sprague-Dawley rats were cultured in vitro, labeled with bisbenzamide (BB) or carboxyfluorescein diacetate (CFDA), and seeded into polycarbonate growth chambers. After 24 h incubation in vitro, the chambers, either empty or containing an arteriovenous (AV) shunt, were implanted subcutaneously into the inguinal region of Sprague-Dawley rats and examined by fluorescence microscopy 2 or 7 days later. The AV shunt remained patent in all experiments. The density of labeled cells on the chamber surface in all chambers decreased in the first 2 days after insertion. At 7 days, the cell density in the empty chambers had not altered from the 2-day ..

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