Journal article

Sequence conservation and antigenic variation of the structural proteins of equine rhinitis A virus

A Varrasso, HE Drummer, JA Huang, RA Stevenson, N Ficorilli, MJ Studdert, CA Hartley

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2001

Abstract

The nucleotide and deduced amino acid sequences of the P1 region of the genomes of 10 independent equine rhinitis A virus (ERAV) isolates were determined and found to be very closely related. A panel of seven monoclonal antibodies to the prototype virus ERAV.393/76 that bound to nonneutralization epitopes conserved among all 10 isolates was raised. In serum neutralization assays, rabbit polyclonal sera and sera from naturally and experimentally infected horses reacted in a consistent and discriminating manner with the 10 isolates, which indicated the existence of variation in the neutralization epitopes of these viruses.