Characterization of the AT4 receptor in a human neuroblastoma cell line (SK-N-MC)

Abstract

Angiotensin IV (Ang IV), the 3-8 fragment of angiotensin II (Ang II), binds to a distinct receptor designated the AT4 receptor. The peptide elicits a range of vascular and central actions including facilitation of memory retention and retrieval in several learning paradigms, The aim of this study was to characterize the AT4 receptor in a human cell line of neural origin. Receptor binding studies indicate that the human neuroblastoma cell line SK-N-MC cells express a high-affinity Ang IV binding site with a pharmacological profile similar to the AT4 receptor: [125I]-Ang IV and [125I]-Nle-1-Ang IV bind specifically to the SK-N-MC cell membranes (Kd = 0.6 and 0.1 nM) in a saturable manner (Bmax..