Journal article

Cytometric and functional analyses of NK and NKT cell deficiencies in NOD mice

LD Poulton, MJ Smyth, CG Hawke, P Silveira, D Shepherd, OV Naidenko, DI Godfrey, AG Baxter

INTERNATIONAL IMMUNOLOGY | OXFORD UNIV PRESS | Published : 2001

Abstract

Defects in NK and NKT cell activities have been implicated in the etiology of type 1 (autoimmune) diabetes in NOD mice on the basis of experiments performed using surrogate phenotypes for the identification of these lymphocyte subsets. Here, we have generated a congenic line of NOD mice (NOD.b-Nkrp1(b)) which express the allelic NK1.1 marker, enabling the direct study of NK and NKT cells in NOD mice. Major deficiencies in both populations were identified when NOD.b-Nkrp1(b) mice were compared with C57BL/6 and BALB.B6-Cmv1(r) mice by flow cytometry. The decrease in numbers of peripheral NK cells was associated with an increase in their numbers in the bone marrow, suggesting that a defect in N..

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University of Melbourne Researchers