Crystallization and preliminary X-ray crystallographic analysis of S-allelic glycoprotein SF11-RNase from Nicotiana alata

Abstract

Nicotiana alata SF11-RNase is an S-glycoprotein associated with gametophytic self-incompatibility. Crystals of SF11-RNase have been grown at room temperature using polyethylene glycol as a precipitant. A crystal diffracted to better than 1.4 Å resolution at 100 K at the SPring-8 synchrotron-radiation source, indicating that it is very suitable for high-resolution structure analysis. The crystal belongs to the space group P21, with unit-cell parameters a = 65.86 (11), b = 44.73 (5), c = 64.36 (7)Å, β = 90.27 (9)°. The asymmetric unit contains two monomers, giving a crystal volume per protein mass (VM) of 2.05 Å3 Da-1 and a solvent content of 39.6% by volume. A full set of X-ray diffraction da..