Journal article

Crystallization and preliminary X-ray crystallographic analysis of S-allelic glycoprotein S-F11-RNase from Nicotiana alata

K Ida, T Shinkawa, S Norioka, E Newbigin, AE Clarke, F Sakiyama, M Sato

Acta Crystallographica Section D - Biological Crystallography | INT UNION CRYSTALLOGRAPHY | Published : 2001


Nicotiana alata S(F11)-RNase is an S-glycoprotein associated with gametophytic self-incompatibility. Crystals of S(F11)-RNase have been grown at room temperature using polyethylene glycol as a precipitant. A crystal diffracted to better than 1.4 A resolution at 100 K at the SPring-8 synchrotron-radiation source, indicating that it is very suitable for high-resolution structure analysis. The crystal belongs to the space group P2(1), with unit-cell parameters a = 65.86 (11), b = 44.73 (5), c = 64.36 (7) A, beta = 90.27 (9) degrees. The asymmetric unit contains two monomers, giving a crystal volume per protein mass (V(M)) of 2.05 A(3) Da(-1) and a solvent content of 39.6% by volume. A full set ..

View full abstract