Journal article

Variable galactocerebroside expression by human Schwann cells in dissociated and peripheral nerve explant cultures

VJ Turnbull, S Petratos, R Papadopoulos, MF Gonzales, M Ayers

JOURNAL OF NEUROSCIENCE RESEARCH | WILEY-LISS | Published : 2001

Abstract

It has been well established that rat Schwann cells down regulate their cell-surface expression of galactocerebroside (GalC) in vitro under normal cell culture conditions. To determine whether human Schwann cells exhibit a similar down-regulation of GalC in vitro we examined GalC expression in dissociated human Schwann cell cultures derived from normal adult peripheral nerve. Twenty-four hours post-dissociation up to 63% of human Schwann cells were found to express detectable levels of GalC on their surface whereas less than 8% of the Schwann cells expressed detectable levels of GalC at 14 days post-dissociation. In contrast, after nearly 3 months of peripheral nerve explant culture, greater..

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University of Melbourne Researchers