Platelet alpha- and gamma-synucleins in Parkinson's disease and normal control subjects
Qiao-Xin Li, Bruce C Campbell, Catriona A McLean, Domimic Thyagarajan, Wei-Ping Gai, Robert M Kapsa, Konrad Beyreuther, Colin L Masters, Janetta G Culvenor
Journal of Alzheimer's Disease | IOS PRESS | Published : 2002
Alpha-synuclein (alphaSN) has been implicated in Parkinson's Disease (PD) and alphaSN is a major component of Lewy bodies (LBs). This study explored platelets as a model system for study of alphaSN metabolism and platelet alphaSN as a diagnostic marker for PD. We used Western blot analysis to characterize and compare platelet and brain alpha-, beta- and gammaSN; and to quantitate alphaSN levels in platelets from PD and age-matched controls. We found that platelets contain full-length alphaSN and 6 and 12 kDa fragments, and gammaSN-like protein. alphaSN and gammaSN were not secreted by thrombin-activated platelets. Furthermore, we also found that the alphaSN and gammaSN levels in sporadic PD ..View full abstract
We thank E. Storey for collecting Parkinson's and age-matched blood. This study is supported by the Australian NHMRC.