Quantitative analysis of in situ hybridization histochemistry

Abstract

Publisher Gene regulation can be studied using a large number of molecular biological techniques. However, only in situ hybridization (ISH) histochemistry permits the precise regional and cellular localization of gene expression in a particular tissue, especially in morphologically and functionally complex tissues such as the nervous system. Because changes in gene transcription, indicated by altered levels of mRNA encoding specific peptides or proteins, are physiologically relevant markers of cellular activity, the ability to perceive these changes and to determine their magnitude accurately is of great importance. When comparing levels of mRNA in different anatomical locations or developme..