Journal article

Targeting of the GRIP domain to the trans-Golgi network is conserved from protists to animals

MJ McConville, SC Ilgoutz, RD Teasdale, BJ Foth, A Matthews, KA Mullin, PA Gleeson

EUROPEAN JOURNAL OF CELL BIOLOGY | URBAN & FISCHER VERLAG | Published : 2002

Abstract

The GRIP domain, found in a family of coiled-coil peripheral membrane Golgi proteins, is a specific targeting sequence for the trans-Golgi network of animal cells. In this study we show that a coiled-coil protein with a GRIP domain occurs in the primitive eukaryote, Trypanosoma brucei, and that reporter proteins containing this domain can be used as a marker for the poorly characterized trans Golgi/trans-Golgi network of trypanosomatid parasites. The T. brucei GRIP domain, when fused to the carboxyl terminus of the green fluorescent protein (GFP-TbGRIP), was efficiently localized to the Golgi apparatus of transfected COS cells. Overexpression of GFP-TbGRIP in COS cells displaced the endogeno..

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