Involvement of T-type calcium channels in excitatory junction potentials in rat resistance mesenteric arteries

Abstract

1. We investigated the role of voltage-operated calcium channels in sympathetic transmission and depolarization-induced contractions in the rat mesenteric artery. In particular, we investigated the role of the T-type voltage-operated calcium channels (T-channels) in mediating excitatory junction potentials (EJPs). 2. EJPs were evoked by electrical field stimulation (trains of five stimuli at 0.9 Hz) in small mesenteric arteries. The average resting membrane potential was -59.8±0.5 mV (n=65). Trains of stimuli evoked individual EJPs with the peak EJP of 6±0.2 mV (n=34) occurring with the second stimulus. Trains of EJPs were inhibited 90% by tetrodotoxin (0.1 μM) or by ω-conotoxin GVIA (GVIA, ..