Journal article

Secretory antibodies do not affect the composition of the bacterial microbiota in the terminal ileum of 10-week-old mice

L Sait, M Galic, RA Strugnell, PH Janssen

Applied and Environmental Microbiology | AMER SOC MICROBIOLOGY | Published : 2003


Terminal restriction fragment length polymorphism (T-RFLP) analysis was conducted on the 16S rRNA genes of the bacterial communities colonizing the epithelial surfaces of the terminal ilea of open conventionally housed mice in an institutional small-animal facility. Polymeric-immunoglobulin-receptor-deficient (pIgR(-/-)) mice that were unable to secrete antibodies across mucosal surfaces were cohoused with normal and otherwise genetically identical wild-type (C57BL/6) mice for 4 weeks. If secretory antibodies played a role in modeling the gastrointestinal microbiota, C57BL/6 mice would have had a more distinct and uniform microbiota than their pIgR(-/-) cage mates. The T-RFLP profiles of the..

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University of Melbourne Researchers