Journal article

Peripheral blood mononuclear cell expression of toll-like receptors and relation to cytokine levels in cirrhosis

SM Riordan, N Skinner, A Nagree, H McCallum, CJ McIver, J Kurtovic, JA Hamilton, S Bengmark, R Williams, K Visvanathan

HEPATOLOGY | WILEY-BLACKWELL | Published : 2003

Abstract

Activation of macrophages by endotoxin is assumed responsible for increased circulating tumor necrosis factor alpha (TNF-alpha) and soluble TNF receptor (sTNFR) levels in cirrhosis. Relevant to this is expression of Toll-like receptor (TLR) 4 and TLR2, which is critically involved in production of TNF-alpha in response to endotoxin and Gram-positive microbial stimuli, respectively. The first studies on this in cirrhosis are reported here. In 36 cirrhotic patients and 32 controls, we measured (1) circulating endotoxin, TNF-alpha, and sTNFR levels; (2) peripheral blood mononuclear cell (PBMC) expression of TLR4 and TLR2, and (3) in vitro TNF-alpha production by PBMCs stimulated with endotoxin ..

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