Journal article

An approach to remove albumin for the proteomic analysis of low abundance biomarkers in human serum

N Ahmed, G Barker, K Oliva, D Garfin, K Talmadge, H Georgiou, M Quinn, G Rice

PROTEOMICS | WILEY | Published : 2003

Abstract

Proteomic technologies are being used to discover and identify disease-associated biomarkers. The application of these technologies in the search for potential diagnostic/prognostic biomarkers in the serum of patients has been limited by the presence of highly abundant albumin and immunoglobulins that constitute approximately 60-97% of the total serum proteins. The purpose of the study was to evaluate whether treatment of human serum with Affi-Gel Blue alone or in combination with Protein A (Aurum serum protein mini kit, Bio-Rad) before two-dimensional gel electrophoresis (2-DE) analysis removed high abundance proteins to allow the visualization of low abundant proteins. Serum samples were t..

View full abstract