Journal article

Neurochemical changes in dopamine D1, D3 and D1/D3 receptor knockout mice

JYF Wong, JJ Clifford, JS Massalas, DI Finkelstein, MK Horne, JL Waddington, J Drago

EUROPEAN JOURNAL OF PHARMACOLOGY | ELSEVIER | Published : 2003

Abstract

Neurochemical changes were examined in dopamine D1 receptor knockout (D1(-/-)), dopamine D3 receptor knockout (D3(-/-)) and dopamine D1/D3 receptor double knockout (D1(-/-)D3(-/-)) mice. The level of dopamine D1- and D2-like receptors and gamma-aminobutyric acid (GABA(A)) receptor was assessed by ligand autoradiography and dopamine D1- and D2 receptor, enkephalin, dynorphin and substance P transcripts measured by in situ hybridization. D1(-/-) mice had normal GABA(A) receptor levels, reduced dynorphin and substance P, and increased enkephalin mRNA and dopamine D2-like binding. D1(-/-)D3(-/-) mice evidenced decreased dynorphin and substance P but normal enkephalin expression, whereas dopamine..

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