Journal article

The effect of pH on the foam fractionation of beta-glucosidase and cellulase

WD Lambert, LP Du, YF Ma, V Loha, V Burapatana, A Prokop, RD Tanner, NB Pamment

BIORESOURCE TECHNOLOGY | ELSEVIER SCI LTD | Published : 2003

Abstract

The surface tension-pH profile of beta-glucosidase was established to determine its relationship to the corresponding profile of cellulase and to the foam fractionation of that cellulase. The goal of this work was to determine the optimal foaming points for both cellulase and cellobiase. This data may prove useful in the separation of certain components of cellulase, since the non-foaming hydrophilic beta-glucosidase does not foam as well as the hydrophobic components of cellulase at low concentrations. A key finding from these experiments was that there are two local minima in the surface tension-pH trajectory for Trichoderma reesei cellulase, as contrasted to the usual single minimum. The ..

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University of Melbourne Researchers