The frequency of the canine leukocyte adhesion deficiency (CLAD) allele within the Irish Setter population of Australia

Abstract

Objective: To determine the frequency of the 107G→C canine leukocyte adhesion deficiency (CLAD) mutation in Irish Setters from the Australian breeding population. Method: Genomic DNA was isolated from 87 Irish Setter blood samples and a region of the p-2 integrin gene (ITGB2), encompassing the mutation, was amplified using real-time Polymerase Chain Reaction (PCR). Two fluorescently labelled probes were hybridised to the fragment, and fluorescence resonance energy transfer (FRET) was used to detect the 107G→C mutation responsible for CLAD. Results: Three new heterozygotes were identified among 87 healthy Irish Setters from Australia. All originated from a litter sired by a known heterozygote..