Preimplantation aneuploidy screening using comparative genomic hybridization or fluorescence in situ hybridization of embryos from patients with recurrent implantation failure

Abstract

Objective: To select chromosomally euploid embryos for transfer by analyzing single biopsied blastomeres using either fluorescence in situ hybridization (FISH) for chromosomes 13, 16, 18, 21, and 22 or comparative genomic hybridization (CGH), which provides a full karyotype. Design: Prospective observational study. Setting: A large IVF unit and the research laboratory of a hospital clinical genetics unit. Patient(s): Twenty patients with recurrent implantation failure. Intervention(s): Ovarian stimulation and IVF by intracytoplasmic sperm injection (ICSI), embryo biopsy, and embryo transfer. Main Outcome Measure(s): Chromosome normality of biopsied blastomeres and implantation and clinical p..