Journal article

Elucidating the ecology of bucephalid parasites using a mutation scanning approach

KS Hutson, CA Styan, I Beveridge, MJ Keough, XQ Zhu, YGA EL-Osta, RB Gasser

MOLECULAR AND CELLULAR PROBES | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2004

Abstract

Nucleotide variation in a portion of the mitochondrial cytochrome c oxidase subunit1 (cox1) gene from asexual stages of bucephalids of southern Australian scallops (Chlamys asperrima, Chlamys bifrons and Pecten fumatus) was investigated using a mutation scanning-sequencing approach. Single-strand conformation polymorphism (SSCP) analysis revealed three main profile types (A, B and C) for parasites isolated from scallops. Sequence analysis revealed that samples represented by profiles B and C had a high degree (97.3%) of sequence similarity, whereas they were approximately 21% different in sequence from those represented by profile A. These findings suggested that at least two types or specie..

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