Journal article

Tracing functional circuits using C-Fos regulated expression of marker genes targeted to neuronal projections

M Murphy, U Greferath, N Nag, J Nithianantharajah, YM Wilson

FRONTIERS IN BIOSCIENCE-LANDMARK | FRONTIERS IN BIOSCIENCE INC | Published : 2004

Abstract

We have developed novel techniques to trace functionally activated circuits and synaptic plasticity within the brain. We have generated transgenic mice, FTL, which contain a tau-lacZ fusion gene regulated by the promoter for c-fos. Following a particular nervous system stimulation in these mice, only neurons, which are functionally activated, will express LacZ, which is targeted to neuronal processes by the tau protein. In the FTL mice, we found highly inducible expression of lacZ by a range of different stimuli, and successful targeting of expression to neuronal cell bodies, axons and dendrites. To test if a functionally activated circuit could be visualized, the mice were deprived of water..

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