Gastric parietal cell acid secretion in mice can be regulated independently of H /K ATPase endocytosis

Abstract

Background & Aims: Gastric parietal cells secrete acid into the lumen of the stomach. They express a proton pump, the gastric H+/K + ATPase, the activity of which is tightly regulated. The H +/K+ ATPase traffics between an intracytoplasmic compartment (tubulovesicles) in quiescent parietal cells and the apical plasma membrane in activated cells. These trafficking events are considered to contribute to the control of acid secretion by modulating access to apical K+ and Cl- conductances that are required for transmembrane H+ ion transport by the H+/K+ ATPase. Here, we have determined whether the control of acid secretion in vivo requires membrane trafficking of the H+/K+ ATPase. Methods: We de..