Journal article

Efficient gene transfer into the epithelial cell layer of embryonic mouse intestine using low-voltage electroporation

HE Abud, P Lock, JK Heath

GASTROENTEROLOGY | W B SAUNDERS CO-ELSEVIER INC | Published : 2004

Abstract

BACKGROUND & AIMS: Gene-targeted and transgenic mice have provided profound insights into the genetic control of intestinal development and differentiation. Here we describe a complementary approach using an innovative embryonic gut culture system in which the tubular architecture of the gut is maintained and rapid analysis of gene function can be achieved by introduction of expression constructs into the epithelial cell layer by using low-voltage square-wave electroporation. METHODS: Electroporation of embryonic gut explants was optimized by using an electrosquareporator to vary the voltage and number of electric pulses. The pulses were applied unidirectionally to confine electroporation of..

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