Journal article

Molecular cloning, expression, and serological evaluation of an, 8-kilodalton subunit of antigen B from Echinococcus multilocularis

W Mamuti, H Yamasaki, Y Sako, M Nakao, N Xiao, K Nakaya, N Sato, DA Vuitton, R Piarroux, MW Lightowlers, PS Craig, A Ito

Journal of Clinical Microbiology | AMER SOC MICROBIOLOGY | Published : 2004

Abstract

Full-length cDNA and genomic DNA encoding an 8-kDa subunit of antigen B from Echinococcus multilocularis (designated EmAgB8/1) were isolated from an E. multilocularis metacestode cDNA library and a protoscolex genomic DNA library, respectively. The open reading frame of the cDNA clone encodes a polypeptide comprising 85 amino acids with a 20-amino-acid NH(2)-terminal signal sequence, which was confirmed following N-terminal sequencing of the native antigen. Reverse transcription-PCR analysis revealed that the clone encoding EmAgB8/1 is predominantly transcribed in larval E. multilocularis. The gene consists of two exons (encoding the signal sequence and mature protein) separated by a 91-bp i..

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University of Melbourne Researchers