Monocarboxylate transport inhibition alters retinal function and cellular amino acid levels

Abstract

We assessed the effect of the in vivo application of monocarboxylate transport inhibitors on retinal function and amino acid immunocytochemistry. We wanted to determine the impact that altered aerobic metabolite availability has on retinal function and the characteristics of amino acid shunting into metabolic pools. Electroretinograms were collected from anaesthetized rats at various times after intravitreal injection of the monocarboxylate transport inhibitors α-cyano-4-hydroxycinnamate (4-CIN; 2 μL, 0.1-10 mM) or p-(dipropylsulphamoyl)benzoic acid (probenecid; 1-10 mM). Changes in retinal function were compared with quantitative amino acid immunocytochemical changes in retinas harvested 20..