Journal article

Early inducible nitric oxide synthase 2 (NOS 2) activity enhances ischaemic skin flap survival.

Sunao Furuta, Peter Vadiveloo, Rosalind Romeo-Meeuw, Wayne Morrison, Alastair Stewart, Geraldine Mitchell

Angiogenesis | Published : 2004


A functional skin-flap model of angiogenesis in the mouse was utilized to investigate ischaemic flap survival/angiogenesis whilst under pharmacological or genetic inhibition of nitric oxide synthase (NOS). In this model, the epigastric artery was cauterized. Following a five-day angiogenic period an abdominal skin-flap supplied by the pre-existing epigastric artery was raised and resutured. After a further six days the outcome was determined by measuring the area of living skin-flap that was sustained by new vessel growth around the cauterized artery. Both pharmacological [ S-methyl-isothiourea (SMT) given via i.p. injection for the five-day angiogenic period] and genetic inhibition of NOS 2..

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