Journal article

Oxidation of low-density lipoproteins induces amyloid-like structures that are recognized by macrophages

CR Stewart, AA Tseng, YF Mok, MK Staples, CH Schiesser, LJ Lawrence, JN Varghese, KJ Moore, GJ Howlett

BIOCHEMISTRY | AMER CHEMICAL SOC | Published : 2005

Abstract

The macrophage scavenger receptor CD36 plays a key role in the initiation of atherosclerosis through its ability to bind to and internalize oxidized low-density lipoproteins (oxLDL). Prompted by recent findings that the CD36 receptor also recognizes amyloid fibrils formed by beta-amyloid and apolipoprotein C-II, we investigated whether the oxidation of low-density lipoproteins (LDL) generates characteristic amyloid-like structures and whether these structures serve as CD36 ligands. Our studies demonstrate that LDL oxidized by copper ions, 2,2-azobis(2-amidinopropane) dihydrochloride (AAPH), or ozone react with the diagnostic amyloid dyes thioflavin T and Congo Red and bind to serum amyloid P..

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