Journal article

Anti sense-mediated suppression of hyaluronan synthase 2 inhibits the tumorigenesis and progression of breast cancer

L Udabage, GR Brownlee, M Waltham, T Blick, EC Walker, P Heldin, SK Nilsson, EW Thompson, TJ Brown

Cancer Research | AMER ASSOC CANCER RESEARCH | Published : 2005

Abstract

The progression of several cancers is correlated with the increased synthesis of the glycosaminoglycan, hyaluronan. Hyaluronan is synthesized at the plasma membrane by various isoforms of hyaluronan synthases (HAS). The importance of HAS2 expression in highly invasive breast cancer was characterized by the antisense inhibition of HAS2 (ASHAS2). The effect of HAS2 inhibition on cell proliferation, migration, hyaluronan metabolism, and receptor status was characterized in vitro, whereas the effect on tumorigenicity and metastasis was established in vivo. HAS2 inhibition resulted in a 24-hour lag in proliferation that was concomitant to transient arrest of 79% of the cell population in G0-G1. I..

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