Journal article

Regulation of the energy sensor AMP-activated protein kinase in the kidney by dietary salt intake and osmolality

Scott Fraser, Peter Mount, Rebecca Hill, Vicki Levidiotis, Frosa Katsis, David Stapleton, Bruce E Kemp, David A Power

American Journal of Physiology: Renal Physiology | American Physiological Society | Published : 2005

Abstract

The AMP-activated protein kinase (AMPK) is a key controller of cellular energy metabolism. We studied its expression and regulation by salt handling in the kidney. Immunoprecipitation and Western blots of protein lysates from whole rat kidney using subunit-specific antibodies showed that the α1-catalytic subunit is expressed in the kidney, associated with the β2- and either γ1- or γ2-subunits. Activated AMPK, detected by immunohistochemical staining for phospho-Thr172 AMPK (pThr172), was expressed on the apical surface of the cortical thick ascending limb of the loop of Henle, including the macula densa, and some parts of the distal convoluted tubule. Activated AMPK was also expressed on the..

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