Journal article

Low TRBP levels support an innate human immunodeficiency virus type 1 resistance in astrocytes by enhancing the PKR antiviral response

CL Ong, JC Thorpe, PR Gorry, S Bannwarth, A Jaworowski, JL Howard, S Chung, S Campbell, HS Christensen, G Clerzius, AJ Mouland, A Gatignol, DFJ Purcell

Journal of Virology | AMER SOC MICROBIOLOGY | Published : 2005

Abstract

Acute human immunodeficiency virus type 1 (HIV-1) replication in astrocytes produces minimal new virus particles due, in part, to inefficient translation of viral structural proteins despite high levels of cytoplasmic viral mRNA. We found that a highly reactive double-stranded (ds) RNA-binding protein kinase (PKR) response in astrocytes underlies this inefficient translation of HIV-1 mRNA. The dsRNA elements made during acute replication of HIV-1 in astrocytes triggers PKR activation and the specific inhibition of HIV-1 protein translation. The heightened PKR response results from relatively low levels of the cellular antagonist of PKR, the TAR RNA binding protein (TRBP). Efficient HIV-1 pro..

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University of Melbourne Researchers