Journal article

High glucose-induced impairment in insulin secretion is associated with reduction in islet glucokinase in a mouse model of susceptibility to islet dysfunction

S Kooptiwut, M Kebede, S Zraika, S Visinoni, K Aston-Mourney, J Favaloro, C Tikellis, MC Thomas, JM Forbes, ME Cooper, M Dunlop, J Proietto, S Andrikopoulos

Journal of Molecular Endocrinology | BIOSCIENTIFICA LTD | Published : 2005

Abstract

Type 2 diabetes is characterized by islet dysfunction resulting in hyperglycemia, which can then lead to further deterioration in islet function. A possible mechanism for hyperglycemia-induced islet dysfunction is the accumulation of advanced glycation end products (AGE). The DBA/2 mouse develops pancreatic islet dysfunction when exposed to a high glucose environment and/or obesity-induced insulin resistance. To determine the biochemical cause of dysfunction, DBA/2 and C57BL/6 control islets were incubated in 11.1 mM or 40 mM glucose in the absence or presence of the AGE inhibitor aminoguanidine (AG) for 10 days. Basal (2.8 mM glucose) insulin release was increased in both DBA/2 and C57BL/6 ..

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